TOol for Motif TO Motif comparison (TOMTOM)

For further information on how to interpret these results or to get a copy of the MEME software please access http://meme.nbcr.net.

If you use TOMTOM in your research, please cite the following paper:
Shobhit Gupta, JA Stamatoyannopolous, Timothy Bailey and William Stafford Noble, "Quantifying similarity between motifs", Genome Biology, 8(2):R24, 2007.

Query Motifs  |  Target Databases  |  Matches  |  Program information  |  Explanation

Query Motifs

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Name 

Preview 

Matches 

List 

1_e_k4me3_8_0.388
CCGCGCGCG
4 RSC30_01_M01520_T,  Rsc30_GR09_U,  RSC30_MA0375.1_J,  ZF5_01_M00716_T
2_e_k36me3_0_0.392
GAATCGCTTGA
0
3_e_k4me3_77_0.398
CGCGGTAC
0
4_e_k36me3_3_0.400
CGCCTGTAA
0
5_h_k4me3_2_0.403
TAATCGTT
0
6_e_k4me3_188_0.405
ACTGCGGT
0
7_e_k36me3_57_0.414
CTGGGCAACA
0
8_e_k36me3_51_0.415
CAGCTACTCA
0
9_e_k36me3_83_0.416
CAGGAGTTCA
1 RPC155_01_M01798_T
10_h_k4me3_4_0.417
TCGCGTTT
0
11_e_k36me3_99_0.418
AGGCTGGTC
2 SMAP1L_4_H,  TRIM21_5_H
12_e_k36me3_28_0.422
AGTTCGAGA
0
13_e_k27me3_11_0.422
CGCAGCGCG
0
14_e_k4me3_67_0.423
AAGATGGCG
1 REX1_03_M01744_T
15_e_k27ac_406_0.427
AAAATGCTG
1 Mafk_primary_SCI09_U
16_e_k4me3_335_0.429
CCGATTTG
0
17_h_k4me3_8_0.435
TCGTAACG
0
18_e_k9me3_393_0.564
TCATGGGG
0
19_e_k36me3_114_0.441
AGGTGTGTG
1 TBX5_Q5_M01044_T
20_e_k36me3_166_0.446
CGCCTTGGC
0
21_e_k36me3_233_0.452
CATCGTGCC
0
22_e_k27me3_66_0.457
AAGAGCGC
0
23_e_k9me3_124_0.542
AGCCAATC
1 NFY_C_M00209_T
24_e_k4me1_434_0.539
TATGTCCCCA
0
25_e_k9me3_390_0.535
ATTTGGGTG
0
26_e_k9me3_61_0.533
GATTGTGAG
0
27_e_k9me3_6_0.531
TGATGATTCC
0
28_e_k9me3_199_0.524
GCATTCAT
0
29_h_k36me3_24_0.519
GCCTCATCATAG
0
30_h_k9me3_4_0.519
AAGAAGGCTTTA
0

Target Databases

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Database 

Number of Motifs 

Motifs Matched 

trnsfcJsprUnprobHpdiCmbned3.meme 2921 11

Matches to Query: 1_e_k4me3_8_0.388

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Summary 

Alignment 

Name RSC30_01_M01520_T
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 9.45761e-07
E-value 0.00276257
q-value 0.00318188
Overlap 9
Offset 5
Orientation Reverse Complement
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Summary 

Alignment 

Name Rsc30_GR09_U
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 1.09398e-06
E-value 0.00319551
q-value 0.00318188
Overlap 9
Offset 5
Orientation Reverse Complement
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Summary 

Alignment 

Name RSC30_MA0375.1_J
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 2.77036e-06
E-value 0.00809222
q-value 0.00322309
Overlap 8
Offset -1
Orientation Normal
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Summary 

Alignment 

Name ZF5_01_M00716_T
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 1.2374e-05
E-value 0.0361446
q-value 0.010283
Overlap 8
Offset 0
Orientation Reverse Complement
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Matches to Query: 2_e_k36me3_0_0.392

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Matches to Query: 3_e_k4me3_77_0.398

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Matches to Query: 4_e_k36me3_3_0.400

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Matches to Query: 5_h_k4me3_2_0.403

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Matches to Query: 6_e_k4me3_188_0.405

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Matches to Query: 7_e_k36me3_57_0.414

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Matches to Query: 8_e_k36me3_51_0.415

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Matches to Query: 9_e_k36me3_83_0.416

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Summary 

Alignment 

Name RPC155_01_M01798_T
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 4.78308e-06
E-value 0.0139714
q-value 0.0279427
Overlap 10
Offset 1
Orientation Normal
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Matches to Query: 10_h_k4me3_4_0.417

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Matches to Query: 11_e_k36me3_99_0.418

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Summary 

Alignment 

Name SMAP1L_4_H
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 2.23938e-06
E-value 0.00654122
q-value 0.0130824
Overlap 6
Offset -1
Orientation Normal
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Summary 

Alignment 

Name TRIM21_5_H
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 9.15642e-06
E-value 0.0267459
q-value 0.0267459
Overlap 6
Offset -1
Orientation Normal
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Matches to Query: 12_e_k36me3_28_0.422

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Matches to Query: 13_e_k27me3_11_0.422

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Matches to Query: 14_e_k4me3_67_0.423

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Summary 

Alignment 

Name REX1_03_M01744_T
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 1.29434e-05
E-value 0.0378077
q-value 0.0756154
Overlap 9
Offset 0
Orientation Normal
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Matches to Query: 15_e_k27ac_406_0.427

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Summary 

Alignment 

Name Mafk_primary_SCI09_U
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 8.58716e-06
E-value 0.0250831
q-value 0.0501662
Overlap 9
Offset 2
Orientation Normal
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Matches to Query: 16_e_k4me3_335_0.429

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Matches to Query: 17_h_k4me3_8_0.435

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Matches to Query: 18_e_k9me3_393_0.564

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Matches to Query: 19_e_k36me3_114_0.441

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Summary 

Alignment 

Name TBX5_Q5_M01044_T
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 1.18949e-06
E-value 0.00347449
q-value 0.00694684
Overlap 9
Offset 1
Orientation Reverse Complement
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Matches to Query: 20_e_k36me3_166_0.446

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Matches to Query: 21_e_k36me3_233_0.452

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Matches to Query: 22_e_k27me3_66_0.457

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Matches to Query: 23_e_k9me3_124_0.542

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Summary 

Alignment 

Name NFY_C_M00209_T
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 6.40332e-06
E-value 0.0187041
q-value 0.0373387
Overlap 8
Offset 3
Orientation Reverse Complement
Create custom LOGO ↧  [Query Top]

Matches to Query: 24_e_k4me1_434_0.539

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Matches to Query: 25_e_k9me3_390_0.535

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Matches to Query: 26_e_k9me3_61_0.533

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Matches to Query: 27_e_k9me3_6_0.531

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Matches to Query: 28_e_k9me3_199_0.524

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Matches to Query: 29_h_k36me3_24_0.519

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Matches to Query: 30_h_k9me3_4_0.519

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TOMTOM version
4.8.1 (Release date: Tue Feb 7 14:03:40 EST 2012)
Reference
Shobhit Gupta, JA Stamatoyannopolous, Timothy Bailey and William Stafford Noble, "Quantifying similarity between motifs", Genome Biology, 8(2):R24, 2007.
Command line summary

Background letter frequencies (from first motif database):
A: 0.295   C: 0.205   G: 0.205   T: 0.295

Result calculation took 65.103 seconds
show model parameters...

Explanation of TOMTOM Results

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The TOMTOM results consist of

Inputs

The important inputs to TOMTOM.

Query Motifs

The query motifs section lists the motifs that were searched for in the target databases as well as links to matches found.

Name
The motif name.
Alt. Name
The alternative motif name. This column may be hidden if no query motifs have an alternate name.
Website
A link to a website which has more information on the motif. This column may be hidden if no query motifs have website information.
Preview
The motif preview. On supporting browsers this will display as a motif logo, otherwise the consensus sequence will be displayed.
Matches
The number of significant matches.
List
Links to the first 20 matches
Target Databases

The target motifs section lists details on the motif databases that were specifed to search.

Database
The database name.
Number of Motifs
The number of motifs read from the motif database minus the number that had to be discarded due to conflicting ids.
Motifs Matched
The number of motifs that had a match with at least one of the query motifs.

Matches

The matches section list the group of motifs that match each query motif. For each query-target match the following information is given:

Summary Table

The summary gives the important statistics about the motif match.

Name
The name of the matched motif.
Alt. Name
The alternative name of the matched motif.
p-value
The probability that the match occurred by random chance according to the null model.
E-value
The expected number of false positives in the matches up to this point.
q-value
The minimum False Discovery Rate required to include the match.
Overlap
The number of letters that overlaped in the optimal alignment.
Offset
The offset of the query motif to the matched motif in the optimal alignment.
Orientation
The orientation of the matched motif that gave the optimal alignment. A value of "normal" means that the matched motif is as it appears in the database otherwise the matched motif has been reverse complemented.
Alignment

The image shows the alignment of the two motifs. The matched motif is shown on the top and the query motif is shown on the bottom.

Create custom LOGO

By clicking the link "Create custom LOGO ↧" a form to make custom logos will be displayed. The download button can then be clicked to generate a motif matching the selected specifications.

Image Type
Two image formats, png and eps, are avaliable. The pixel based portable network graphic (png) format is commonly used on the Internet and the Encapsulated PostScript (eps) format is more suitable for publications that might require scaling.
Error bars
Toggle error bars indicating the confidence of a motif based on the number of sites used in its creation.
SSC
Toggle adding pseudocounts for Small Sample Correction.
Flip
Toggle a full reverse complement of the alignment.
Width
Specify the width of the generated motif.
Height
Specify the height of the generated motif.