TOol for Motif TO Motif comparison (TOMTOM)

For further information on how to interpret these results or to get a copy of the MEME software please access http://meme.nbcr.net.

If you use TOMTOM in your research, please cite the following paper:
Shobhit Gupta, JA Stamatoyannopolous, Timothy Bailey and William Stafford Noble, "Quantifying similarity between motifs", Genome Biology, 8(2):R24, 2007.

Query Motifs  |  Target Databases  |  Matches  |  Program information  |  Explanation

Query Motifs

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Name 

Preview 

Matches 

List 

1_h_k9me3-h1_0.576
GCTCCCAGCGTG
0
2_e_k9me3-h1_0.576
CAGCGTGAGG
0
3_h_k9me3-h1_0.564
TGCACTGATC
0
4_e_k9me3-h1_0.560
CCGGGTTCA
0
5_e_k9me3-h1_0.556
GTCAGGAGA
0
6_e_k9me3-msc_0.550
CATTCATTCT
0
7_e_k9me3-h1_0.549
TGGCTGACA
0
8_e_k9me3-h1_0.548
GGAGTCTCAG
0
9_e_k9me3-msc_0.548
CATCCTTGAA
0
10_e_k9me3-h1_0.546
CTCAGCTTC
0
11_e_k9me3-msc_0.545
TACAGGAAATG
1 FEV_MA0156.1_J
12_e_k9me3-msc_0.545
CCTAAAGCA
0
13_e_k9me3-msc_0.543
CACATTCACC
0
14_e_k9me3-h1_0.543
CTGCGTCGCT
0
15_h_k9me3-msc_0.542
TCCACGGAGA
0
16_h_k9me3-msc_0.542
GGTCCTTC
0
17_h_k9me3-msc_0.541
CTTCGCTG
0
18_e_k9me3-h1_0.541
ACCGCGCCT
0
19_e_k9me3-msc_0.541
CACGTAAGA
0
20_e_k9me3-msc_0.541
AGTTTCCTC
0
21_h_k9me3-msc_0.540
TGCTGCTCATTG
0
22_e_k9me3-msc_0.539
TCTCCTCTA
0
23_e_k9me3-msc_0.539
AAAGCCTTC
0
24_e_k9me3-msc_0.539
TAAGCAGGACG
0
25_e_k9me3-msc_0.538
AACGACTG
0
26_e_k9me3-msc_0.538
CCCTGAAAG
0
27_e_k9me3-msc_0.538
AAAACACT
0
28_e_k9me3-msc_0.538
TACGCTACT
0
29_h_k9me3-msc_0.537
AATCTGTG
0
30_e_k9me3-h1_0.537
GGTGACAGA
0
31_e_k9me3-msc_0.537
ACGATTCTA
0
32_h_k9me3-msc_0.537
CGGAAACA
0
33_e_k9me3-msc_0.535
GGTAAAAAC
0
34_e_k9me3-h1_0.535
ATATGGTG
0
35_e_k9me3-msc_0.535
CGCTAAGAC
0
36_e_k9me3-h1_0.535
GGAGCTGTA
0
37_e_k9me3-h1_0.534
GGATCATGC
0
38_e_k9me3-msc_0.534
ATTAGAACG
0
39_e_k9me3-msc_0.533
CAACAACG
0
40_e_k9me3-msc_0.533
AGACAGACA
1 SMAD3_Q6_M00701_T
41_e_k9me3-h1_0.532
GTGAGATGA
0
42_e_k9me3-msc_0.532
GGCATTTGC
0
43_e_k9me3-msc_0.532
GCTTTCCCA
0
44_e_k9me3-h1_0.531
CCTGAGCTCG
0
45_e_k9me3-h1_0.531
CGCTCCAAA
0
46_e_k9me3-msc_0.530
GAAAAGGTAG
0
47_e_k9me3-h1_0.530
CCCACACCAGG
0
48_e_k9me3-msc_0.530
CGGAATTAG
0
49_e_k9me3-h1_0.529
GAAATCACCC
0
50_e_k9me3-msc_0.528
GCACATTAC
0
51_e_k9me3-h1_0.528
AGGTACCGGG
0
52_e_k9me3-msc_0.527
GGCCTCATT
0
53_e_k9me3-msc_0.523
AAACCTCATT
2 POU6F1_02_M01462_T,  Pou6f1_3733.1_Cell08_U
54_h_k9me3-h1_0.521
ATAAAAACTA
0
55_e_k9me3-msc_0.520
AAACTGCCG
0
56_e_k9me3-h1_0.517
AAAGGGGTC
0
57_e_k9me3-msc_0.517
CAGCCACAC
0
58_e_k9me3-h1_0.510
CCAAATATC
0
59_e_k9me3-h1_0.508
AGAGGTTTA
0
60_e_k9me3-h1_0.507
AGGGTCCTA
0

Target Databases

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Database 

Number of Motifs 

Motifs Matched 

trnsfcJsprUnprobHpdiCmbned3.meme 2921 4

Matches to Query: 1_h_k9me3-h1_0.576

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Matches to Query: 2_e_k9me3-h1_0.576

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Matches to Query: 3_h_k9me3-h1_0.564

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Matches to Query: 4_e_k9me3-h1_0.560

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Matches to Query: 5_e_k9me3-h1_0.556

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Matches to Query: 6_e_k9me3-msc_0.550

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Matches to Query: 7_e_k9me3-h1_0.549

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Matches to Query: 8_e_k9me3-h1_0.548

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Matches to Query: 9_e_k9me3-msc_0.548

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Matches to Query: 10_e_k9me3-h1_0.546

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Matches to Query: 11_e_k9me3-msc_0.545

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Summary 

Alignment 

Name FEV_MA0156.1_J
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 8.1768e-06
E-value 0.0238844
q-value 0.0473268
Overlap 8
Offset -2
Orientation Normal
Create custom LOGO ↧  [Query Top]

Matches to Query: 12_e_k9me3-msc_0.545

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Matches to Query: 13_e_k9me3-msc_0.543

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Matches to Query: 14_e_k9me3-h1_0.543

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Matches to Query: 15_h_k9me3-msc_0.542

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Matches to Query: 16_h_k9me3-msc_0.542

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Matches to Query: 17_h_k9me3-msc_0.541

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Matches to Query: 18_e_k9me3-h1_0.541

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Matches to Query: 19_e_k9me3-msc_0.541

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Matches to Query: 20_e_k9me3-msc_0.541

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Matches to Query: 21_h_k9me3-msc_0.540

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Matches to Query: 22_e_k9me3-msc_0.539

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Matches to Query: 23_e_k9me3-msc_0.539

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Matches to Query: 24_e_k9me3-msc_0.539

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Matches to Query: 25_e_k9me3-msc_0.538

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Matches to Query: 26_e_k9me3-msc_0.538

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Matches to Query: 27_e_k9me3-msc_0.538

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Matches to Query: 28_e_k9me3-msc_0.538

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Matches to Query: 29_h_k9me3-msc_0.537

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Matches to Query: 30_e_k9me3-h1_0.537

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Matches to Query: 31_e_k9me3-msc_0.537

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Matches to Query: 32_h_k9me3-msc_0.537

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Matches to Query: 33_e_k9me3-msc_0.535

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Matches to Query: 34_e_k9me3-h1_0.535

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Matches to Query: 35_e_k9me3-msc_0.535

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Matches to Query: 36_e_k9me3-h1_0.535

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Matches to Query: 37_e_k9me3-h1_0.534

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Matches to Query: 38_e_k9me3-msc_0.534

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Matches to Query: 39_e_k9me3-msc_0.533

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Matches to Query: 40_e_k9me3-msc_0.533

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Summary 

Alignment 

Name SMAD3_Q6_M00701_T
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 5.46992e-08
E-value 0.000159777
q-value 0.000319553
Overlap 9
Offset 0
Orientation Reverse Complement
Create custom LOGO ↧  [Query Top]

Matches to Query: 41_e_k9me3-h1_0.532

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Matches to Query: 42_e_k9me3-msc_0.532

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Matches to Query: 43_e_k9me3-msc_0.532

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Matches to Query: 44_e_k9me3-h1_0.531

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Matches to Query: 45_e_k9me3-h1_0.531

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Matches to Query: 46_e_k9me3-msc_0.530

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Matches to Query: 47_e_k9me3-h1_0.530

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Matches to Query: 48_e_k9me3-msc_0.530

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Matches to Query: 49_e_k9me3-h1_0.529

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Matches to Query: 50_e_k9me3-msc_0.528

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Matches to Query: 51_e_k9me3-h1_0.528

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Matches to Query: 52_e_k9me3-msc_0.527

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Matches to Query: 53_e_k9me3-msc_0.523

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Summary 

Alignment 

Name POU6F1_02_M01462_T
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 2.11059e-06
E-value 0.00616504
q-value 0.00616504
Overlap 10
Offset 1
Orientation Reverse Complement
Create custom LOGO ↧  [Next Match] [Query Top]

Summary 

Alignment 

Name Pou6f1_3733.1_Cell08_U
Database trnsfcJsprUnprobHpdiCmbned3.meme
p-value 2.11059e-06
E-value 0.00616504
q-value 0.00616504
Overlap 10
Offset 1
Orientation Reverse Complement
Create custom LOGO ↧ [Previous Match] [Query Top]

Matches to Query: 54_h_k9me3-h1_0.521

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Matches to Query: 55_e_k9me3-msc_0.520

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Matches to Query: 56_e_k9me3-h1_0.517

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Matches to Query: 57_e_k9me3-msc_0.517

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Matches to Query: 58_e_k9me3-h1_0.510

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Matches to Query: 59_e_k9me3-h1_0.508

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Matches to Query: 60_e_k9me3-h1_0.507

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TOMTOM version
4.8.1 (Release date: Tue Feb 7 14:03:40 EST 2012)
Reference
Shobhit Gupta, JA Stamatoyannopolous, Timothy Bailey and William Stafford Noble, "Quantifying similarity between motifs", Genome Biology, 8(2):R24, 2007.
Command line summary

Background letter frequencies (from first motif database):
A: 0.295   C: 0.205   G: 0.205   T: 0.295

Result calculation took 136.555 seconds
show model parameters...

Explanation of TOMTOM Results

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The TOMTOM results consist of

Inputs

The important inputs to TOMTOM.

Query Motifs

The query motifs section lists the motifs that were searched for in the target databases as well as links to matches found.

Name
The motif name.
Alt. Name
The alternative motif name. This column may be hidden if no query motifs have an alternate name.
Website
A link to a website which has more information on the motif. This column may be hidden if no query motifs have website information.
Preview
The motif preview. On supporting browsers this will display as a motif logo, otherwise the consensus sequence will be displayed.
Matches
The number of significant matches.
List
Links to the first 20 matches
Target Databases

The target motifs section lists details on the motif databases that were specifed to search.

Database
The database name.
Number of Motifs
The number of motifs read from the motif database minus the number that had to be discarded due to conflicting ids.
Motifs Matched
The number of motifs that had a match with at least one of the query motifs.

Matches

The matches section list the group of motifs that match each query motif. For each query-target match the following information is given:

Summary Table

The summary gives the important statistics about the motif match.

Name
The name of the matched motif.
Alt. Name
The alternative name of the matched motif.
p-value
The probability that the match occurred by random chance according to the null model.
E-value
The expected number of false positives in the matches up to this point.
q-value
The minimum False Discovery Rate required to include the match.
Overlap
The number of letters that overlaped in the optimal alignment.
Offset
The offset of the query motif to the matched motif in the optimal alignment.
Orientation
The orientation of the matched motif that gave the optimal alignment. A value of "normal" means that the matched motif is as it appears in the database otherwise the matched motif has been reverse complemented.
Alignment

The image shows the alignment of the two motifs. The matched motif is shown on the top and the query motif is shown on the bottom.

Create custom LOGO

By clicking the link "Create custom LOGO ↧" a form to make custom logos will be displayed. The download button can then be clicked to generate a motif matching the selected specifications.

Image Type
Two image formats, png and eps, are avaliable. The pixel based portable network graphic (png) format is commonly used on the Internet and the Encapsulated PostScript (eps) format is more suitable for publications that might require scaling.
Error bars
Toggle error bars indicating the confidence of a motif based on the number of sites used in its creation.
SSC
Toggle adding pseudocounts for Small Sample Correction.
Flip
Toggle a full reverse complement of the alignment.
Width
Specify the width of the generated motif.
Height
Specify the height of the generated motif.